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Isolation And Purification Of Plant Pathogens-Citrus Canker

Isolation And Purification Of Plant Pathogens-Citrus Canker Pierce the margin of an isolated canker lesion on a citrus leaf with an isolated alcohol-flamed dissecting needle. From each tomato sample select, young lesions cut out a small piece of the tissue using an alcohol-flamed sharp scalpel. Use alcohol-flamed tweezers to transfer the excised tissue into an empty Petri dish containing a 2.5 µl drop of sterile tap water. Carefully macerate the tissue using an alcohol-flamed sterile scalpel and tweezer to release the Bacteria. Use a flamed inoculating loop to transfer a small amount of the bacterial suspension onto a Petri plate containing NA Medium. Subsequently perform a quadrant streak on the plate making sure to flame and cool the inoculating loop before each streak. Label the plates with your name and sample type and place the plates upside down in the 28Ĉ incubator. Isolation And Purification Of Plant Pathogens-Citrus Canker ----------------------------------

Isolation And Purification Of Plant Pathogens-Bacteria (Plant Care)

Isolation And Purification Of Plant Pathogens-Bacteria Purification Xanthomonas spp. Isolation And Purification Of Plant Pathogens-Bacteria Obtain specimens and record the appearance of the tissue and bacterial lesions for the tomato plants inoculated with Xanthomonas vesicatoria cause the tomato lesions. From each tomato sample select, young lesions cut a small piece of the tissue using an alcohol-flamed sharp scalpel. Use alcohol-flamed tweezers to transfer the excised tissue into an empty Petri dish containing a 2.5 µl drop of sterile tap water. Carefully macerate the tissue using an alcohol-flamed sterile scalpel and tweezer to release the Bacteria. Use a flamed inoculating loop to transfer a small amount of the bacterial suspension onto a Petri plate containing NA Medium. Subsequently perform a quadrant streak on the plate making sure to flame and cool the inoculating loop before each streak. Label the plates with your name and sample type and place the p

GEL Procedure Of Gel Electrophoresis For Separation DNA/RNA

GEL Procedure Of Gel Electrophoresis For Separation-DNA/RNA GEL ELECTROPHORESIS GEL Procedure Of Gel Electrophoresis For Separation-DNA/RNA Mix 1 g agarose in 99mL of TAE/TBE buffer and heat it in the oven. Add 10 uL of 10mg/mL ethidium bromide in 100 mL of hand cold agarose gel solution. Pour this mixture into gel caster/tray having comb and sealed from sides. Let it be solidified at room temperature for 30-60 minutes. Remove the comb seals from the gel caster and place it inside the gel tank containing TAE/TBE buffer. Put the DNA samples/PCR product (mixed in 6x loading dye) inside each well and note it in the lab. Notebook. Attach the cathode (towards wells) end of gel and anode (at the bottom) of the power supply to the gel tank. Switch on the power supply giving 80-120 volts and run it for sufficient time allowing the samples to travel in more than half of the gel length. Take the gel out of the gel tank and observe it in a gel doc system/UV transillumin

Recipes For Semi Selective Media For Pathogens Growth

Recipes For Semi Selective Media For Pathogens Growth SM-1 medium (18) to TTC medium add the following after autoclaving; For Litter of Solution (1000ml) Merthiolate tincture 5 to 50 µl Crystal violet 50mg Polymyxin 𝛽 sulfate 100mg Tyrothricin 20mg Chloromycetin 5mg Cycloheximide 50mg **Dissolve in 5ml of 75% ethanol 30 min prior to use. Recipes For Semi Selective Media For Pathogens Growth Modified SMSA medium (12-14) Crystal violet 1% 0.5 ml Polymyxin 𝛽 sulfate 1% 10.0 ml Bacitracin 1% 2.5 ml Chloromycetin 1% 0.5 ml Penicillin 0.1% 0.5 ml Cycloheximide 1% in 70% ethanol 2.5 ml Recipes For Semi Selective Media For Pathogens Growth. ------------------------------------------------------------------------------------------ Here You Can Find Plant Disease and

Media Recipes For Pathogens (Bacterial) Growth On Artificial Mediums

Media Recipes For Pathogens Growth On Artificial Mediums CPG (1L) CPG (For One Litter Solution) Differential media for Ralstonia from laboratory guide for identification of Plant Pathogenic Bacteria 1.0g Casamino acid (casein hydrolysate) 10.0g Peptone 5.0g Glucose 17.0g Agar Media Recipes For Pathogens Growth On Artificial Mediums YNA (Yeast extract nutrient agar) - (For 1L) Differential media for Xanthamonas from laboratory guide for identification of Plant Pathogenic Bacteria 5.0g Yeast extract 23.0g Nutrient agar NA (Nutrient agar) (1L) Media Recipes For Pathogens Growth On Artificial Mediums Cultivation of a wide variety of Microorganisms from Difco Nutrient Agar container 23.0g Nutrient Agar 1/4 PDA (QPDA) (Potato Dextrose Agar) (1L) 9.75g Potato Dextrose Agar 11.25g Agar PDA (Potato Dextrose Agar) (1L) Media Recipes For Pathogens Growth On Artificial Mediums 39g Potato Dextrose Agar

Generalized Life Cycle of Basidiomycota-Plant Care

GENERALIZED LIFE CYCLE OF BASIDIOMYCOTA Basidiomycota is one of two large phyla that, together with the Ascomycota, comprise the subkingdom Dikarya (often referred to as the "higher fungi") within the kingdom Fungi. Basically, Basidiomycota are filamentous fungi composed of hyphae (except for yeasts), and reproducing sexually via the formation of specialized club- shaped end cells called basidia that normally bear external meiospores (usually four). These specialized spores are called basidiospores. (Edited from Google images). ------------------------------------------------------------------------------------------ Here You Can Find Plant Disease and Pathogens (Fungi, Bacteria, Molllicutes, Parasitic higher plants, Parasitic Green Algae, Nematodes, Protozoa, Viruses, Viroids,Prions) Diagnosis and Treatment, Control Against Plant Disease. Laboratory Experiments & Work. Plant Pathology Lab Manual. House Plant-Trees Care Guide, Snake Plant Care, Pot