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Media Recipes For Pathogens (Bacterial) Growth On Artificial Mediums

Media Recipes For Pathogens Growth On Artificial Mediums

Media Recipes For Pathogens Growth On Artificial Mediums

  • CPG (1L)
CPG (For One Litter Solution)
Differential media for Ralstonia from laboratory guide for identification of Plant Pathogenic Bacteria
  1. 1.0g      Casamino acid (casein hydrolysate)
  2. 10.0g    Peptone
  3. 5.0g      Glucose
  4. 17.0g    Agar
Media Recipes For Pathogens Growth On Artificial Mediums
  • YNA (Yeast extract nutrient agar) - (For 1L)
Differential media for Xanthamonas from laboratory guide for identification of Plant Pathogenic Bacteria
  1. 5.0g     Yeast extract
  2. 23.0g   Nutrient agar
  • NA (Nutrient agar) (1L)
Media Recipes For Pathogens Growth On Artificial Mediums
Media Recipes For Pathogens Growth On Artificial Mediums

Cultivation of a wide variety of Microorganisms from Difco Nutrient Agar container
  1. 23.0g      Nutrient Agar
  • 1/4 PDA (QPDA) (Potato Dextrose Agar) (1L)
  1. 9.75g   Potato Dextrose Agar
  2. 11.25g  Agar
  • PDA (Potato Dextrose Agar) (1L) Media Recipes For Pathogens Growth On Artificial Mediums
  1. 39g  Potato Dextrose Agar
  • CPG and TTC media (1L)
  1. Casamine acid (casein hydrolysate)        1.0g
  2. Peptpne                                                    10.0g
  3. Glucose                                                   5.0g
  4. Agar                                                        17.0g
CPG contains the four ingredients shown. To make TTC medium cool the medium to 55 ℃ and add 5 ml of a 1% stock solution of 2,3,5-triphenyl tetrazolium chloride. The stock can be filter sterilized or autoclaved for 5 mint at  121℃ and stored at 4℃ or frozen.
Media Recipes For Pathogens Growth On Artificial Mediums
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Written By:
Dr.Qaiser Shakeel
Tahir Mahmood
Department of Plant Pathology-Agriculture
The Islamia University of Bahwalpur-Pakistan.
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