PREPARATION OF MEDIA FOR CULTURE: Media play an important role not only for the nutrition and growth of the pathogen but also in identification through growth pattern on media. Fungi and Bacteria need synthetic media enriched with essential element for the growth. In general different types of media are prepared to culture fungi and bacteria but Potato dextrose agar (PDA) for fungi and Nutrient agar for bacteria are being used commonly. RECIPES OF DIFFERENT MEDIA: POTATO DEXTROSE AGAR: Potato Starch 250 mL/15g Dextrose-15g Agar -Agar 15g Distilled water 750 mL/1000mL PROCEDURE TO PREPARE POTATO DEXTROSE AGAR MATERIAL: Petri dish, Flask, test tubes, Agar-agar, Dextrose, Potato starch, Water PROCEDURE: First of all take potatoes to extract potato starch. Peel the potatoes and cut into small pieces. Preferably 200 g of potato pieces are weighed, washed quickly in running water, placed in 1l liter of water and boil...
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