Skip to main content

Preparation of Media For Culture-PDA, PDB & NA Recipes

Preparation of Media For Culture-PDA, PDB & NA Recipes

PREPARATION OF MEDIA FOR CULTURE:
Media play an important role not only for the nutrition and growth of the pathogen but also in identification through growth pattern on media. Fungi and Bacteria need synthetic media enriched with essential element for the growth. In general different types of media are prepared to culture fungi and bacteria but Potato dextrose agar (PDA) for fungi and Nutrient agar for bacteria are being used commonly. 
RECIPES OF DIFFERENT MEDIA:
POTATO DEXTROSE AGAR:
Potato Starch 250 mL/15g
Dextrose-15g
Agar -Agar 15g
Distilled water 750 mL/1000mL
Preparation of Media For Culture-PDA, PDB & NA Recipes-Plant Care

PROCEDURE TO PREPARE POTATO DEXTROSE AGAR MATERIAL:
Petri dish, Flask, test tubes, Agar-agar, Dextrose, Potato starch, Water
PROCEDURE:
First of all take potatoes to extract potato starch. Peel the potatoes and cut into small pieces. Preferably 200 g of potato pieces are weighed, washed quickly in running water, placed in 1l liter of water and boiled for nearly 1 hour till a mash is formed. The mash is then squeezed through double layer of muslin cloth to obtain as much of the pulp as possible. Discard the potato pieces. Alternatively potato starch commercially available in the markets can also be used. Take the potato starch 250 mL, add dextrose (15g) into it and slightly shake it so that dextrose completely mixes into starch. Now slowly add agar-agar (15g) into this and continue shaking until the whole contents get mixed thoroughly. Now add the distilled water (750 mL) to make up the volume as per requirement. Plug in the flask and autoclave. After autoclaving, medium is ready to pour into Petri dishes.
Preparation of Media For Culture-PDA, PDB & NA Recipes-Plant Care

POTATO DEXTROSE BROTH:
Potato starch 250ml/15g
Dextrose  15g
Distilled water 750ml/1000ml
PROCEDURE TO PREPARE POTATO DEXTROSE BROTH MATERIAL:
Petri dish, Flask, test tubes, Dextrose, Potato starch, Water
PROCEDURE:
First of all take potatoes to extract potato starch. Peel the potatoes and cut into small pieces. Preferably 200 g of potato pieces are weighed, washed quickly in running water, placed in 11 liter of water and boiled for nearly 1 hour till a mash is formed. The mash is then squeezed through double layer of muslin cloth to obtain as much of the pulp as possible. Discard the potato pieces. Alternatively potato starch commercially available in the markets can also be used. Take the potato starch 250 mL, add dextrose (15g) into it and slightly shake it so that dextrose completely mixes into starch. Now add the distilled water (750 mL) to make up the volume as per requirement. Plug in the flask and autoclave. After autoclaving, medium is ready to pour into Petri dishes.
------------------------------------------------------------------------------------------
Here You Can Find Plant Disease and Pathogens (Fungi, Bacteria, Molllicutes, Parasitic higher plants, Parasitic Green Algae, Nematodes, Protozoa, Viruses, Viroids,Prions) Diagnosis and Treatment, Control Against Plant Disease. Laboratory Experiments & Work. Plant Pathology Lab Manual. House Plant-Trees Care Guide, Snake Plant Care, Pothos Plant Care, Plant Care for dummies, Plant Care  Printable, Products, Plant Care Tips, Symbols, Tags, Database, Careers, Websites, Apps & Card.
Written By:
Dr.Qaiser Shakeel
Tahir Mahmood
Department of Plant Pathology-Agriculture

The Islamia University of Bahwalpur-Pakistan.
Labels:

Comments

Post a Comment

Popular posts from this blog

GEL Procedure Of Gel Electrophoresis For Separation DNA/RNA

GEL Procedure Of Gel Electrophoresis For Separation-DNA/RNA GEL ELECTROPHORESIS GEL Procedure Of Gel Electrophoresis For Separation-DNA/RNA Mix 1 g agarose in 99mL of TAE/TBE buffer and heat it in the oven. Add 10 uL of 10mg/mL ethidium bromide in 100 mL of hand cold agarose gel solution. Pour this mixture into gel caster/tray having comb and sealed from sides. Let it be solidified at room temperature for 30-60 minutes. Remove the comb seals from the gel caster and place it inside the gel tank containing TAE/TBE buffer. Put the DNA samples/PCR product (mixed in 6x loading dye) inside each well and note it in the lab. Notebook. Attach the cathode (towards wells) end of gel and anode (at the bottom) of the power supply to the gel tank. Switch on the power supply giving 80-120 volts and run it for sufficient time allowing the samples to travel in more than half of the gel length. Take the gel out of the gel tank and observe it in a gel doc system/UV transillumin...
Post a Comment
Read more

Generalized Life Cycle Of Plant Pathogenic Bacteria-Plant Care

Generalized Life Cycle Of Plant Pathogenic Bacteria-Plant Care GENERALIZED LIFE CYCLE OF PLANT PATHOGENIC  BACTERIA: Bacteria are the prokaryotes, unicellular microorganisms having DNA as genetic material not bounded by membrane. Plant pathogenic bacteria are rod like; diameter ranging from 0.6-3.5 μm. Different forms of flagella may be present on the body of bacteria. Bacteria reproduce asexually through binary fission or fission at rapid rate. Identification of plant pathogenic bacteria is based on visual observation including symptoms expression, colony pattern on media, microscopic observation, gram staining, serological and molecular techniques. Plant pathogenic bacteria though cause limited number of diseases in plants but all the diseases are of much economic importance. Soft rot, blight and bacterial canker of vegetable and stone fruits are important bacterial diseases. -------------------------------------------------------------------------------------...
Post a Comment
Read more