Isolation And Purification Of Plant Pathogens-Citrus Canker

Isolation And Purification Of Plant Pathogens-Citrus Canker

Pierce the margin of an isolated canker lesion on a citrus leaf with an isolated alcohol-flamed dissecting needle.
From each tomato sample select, young lesions cut out a small piece of the tissue using an alcohol-flamed sharp scalpel.
Use alcohol-flamed tweezers to transfer the excised tissue into an empty Petri dish containing a 2.5 µl drop of sterile tap water.
Carefully macerate the tissue using an alcohol-flamed sterile scalpel and tweezer to release the Bacteria.
Use a flamed inoculating loop to transfer a small amount of the bacterial suspension onto a Petri plate containing NA Medium.
Subsequently perform a quadrant streak on the plate making sure to flame and cool the inoculating loop before each streak.
Label the plates with your name and sample type and place the plates upside down in the 28Ĉ incubator.
Isolation And Purification Of Plant Pathogens-Citrus Canker

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Written By:

Dr.Qaiser Shakeel

Tahir Mahmood

Department of Plant Pathology-Agriculture

The Islamia University of Bahwalpur-Pakistan.

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