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Recipes For Semi Selective Media For Pathogens Growth

 Recipes For Semi selective Media For Pathogens Growth In Laboratory

 Recipes For Semi Selective Media For Pathogens Growth
  • SM-1 medium (18) to TTC medium add the following after autoclaving; 
  • For Litter of Solution (1000ml)
  1. Merthiolate tincture               5 to 50 µl
  2. Crystal violet                         50mg
  3. Polymyxin 𝛽 sulfate              100mg
  4. Tyrothricin                            20mg
  5. Chloromycetin                      5mg 
  6. Cycloheximide                     50mg
**Dissolve in 5ml of 75% ethanol 30 min prior to use.
 Recipes For Semi Selective Media For Pathogens Growth
 Recipes For Semi Selective Media For Pathogens Growth
  • Modified SMSA medium (12-14)
  1. Crystal violet 1%                0.5 ml
  2. Polymyxin 𝛽 sulfate  1%    10.0 ml
  3. Bacitracin 1%                     2.5 ml
  4. Chloromycetin 1%            0.5 ml
  5. Penicillin  0.1%                 0.5 ml
  6. Cycloheximide 1% in 70% ethanol 2.5 ml
 Recipes For Semi Selective Media For Pathogens Growth.
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Written By:
Dr.Qaiser Shakeel
Tahir Mahmood
Department of Plant Pathology-Agriculture
The Islamia University of Bahwalpur-Pakistan.

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