Recipes For Semi Selective Media For Pathogens Growth

 Recipes For Semi Selective Media For Pathogens Growth

• SM-1 medium (18) to TTC medium add the following after autoclaving; 
• For Litter of Solution (1000ml)

1. Merthiolate tincture               5 to 50 µl
2. Crystal violet                         50mg
3. Polymyxin 𝛽 sulfate              100mg
4. Tyrothricin                            20mg
5. Chloromycetin                      5mg 
6. Cycloheximide                     50mg

**Dissolve in 5ml of 75% ethanol 30 min prior to use.

 Recipes For Semi Selective Media For Pathogens Growth

• Modified SMSA medium (12-14)

1. Crystal violet 1%                0.5 ml
2. Polymyxin 𝛽 sulfate  1%    10.0 ml
3. Bacitracin 1%                     2.5 ml
4. Chloromycetin 1%            0.5 ml
5. Penicillin  0.1%                 0.5 ml
6. Cycloheximide 1% in 70% ethanol 2.5 ml

 Recipes For Semi Selective Media For Pathogens Growth.

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Written By:

Dr.Qaiser Shakeel

Tahir Mahmood

Department of Plant Pathology-Agriculture

The Islamia University of Bahwalpur-Pakistan.