GEL Procedure Of Gel Electrophoresis For Separation DNA/RNA

GEL Procedure Of Gel Electrophoresis For Separation-DNA/RNA

• GEL ELECTROPHORESIS

GEL Procedure Of Gel Electrophoresis For Separation-DNA/RNA

• Mix 1 g agarose in 99mL of TAE/TBE buffer and heat it in the oven.
• Add 10 uL of 10mg/mL ethidium bromide in 100 mL of hand cold agarose gel solution.
• Pour this mixture into gel caster/tray having comb and sealed from sides.
• Let it be solidified at room temperature for 30-60 minutes.
• Remove the comb seals from the gel caster and place it inside the gel tank containing TAE/TBE buffer.
• Put the DNA samples/PCR product (mixed in 6x loading dye) inside each well and note it in the lab.
• Notebook.
• Attach the cathode (towards wells) end of gel and anode (at the bottom) of the power supply to the gel tank.
• Switch on the power supply giving 80-120 volts and run it for sufficient time allowing the
• samples to travel in more than half of the gel length.
• Take the gel out of the gel tank and observe it in a gel doc system/UV transilluminator for
• visualization of relevant bands.

GEL Procedure Of Gel Electrophoresis For Separation-DNA/RNA

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Written By:

Dr.Qaiser Shakeel

Tahir Mahmood

Department of Plant Pathology-Agriculture

The Islamia University of Bahwalpur-Pakistan.