Isolation And Purification Of Plant Pathogens-Bacteria (Plant Care)

Isolation And Purification Of Plant Pathogens-Bacteria

Purification Xanthomonas spp.

Isolation And Purification Of Plant Pathogens-Bacteria

Obtain specimens and record the appearance of the tissue and bacterial lesions for the tomato plants inoculated with Xanthomonas vesicatoria cause the tomato lesions.
From each tomato sample select, young lesions cut a small piece of the tissue using an alcohol-flamed sharp scalpel.
Use alcohol-flamed tweezers to transfer the excised tissue into an empty Petri dish containing a 2.5 µl drop of sterile tap water.
Carefully macerate the tissue using an alcohol-flamed sterile scalpel and tweezer to release the Bacteria.
Use a flamed inoculating loop to transfer a small amount of the bacterial suspension onto a Petri plate containing NA Medium.
Subsequently perform a quadrant streak on the plate making sure to flame and cool the inoculating loop before each streak.
Label the plates with your name and sample type and place the plates upside down in the 28Ĉ incubator.
Isolation And Purification Of Plant Pathogens-Bacteria

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Written By:

Dr.Qaiser Shakeel

Tahir Mahmood

Department of Plant Pathology-Agriculture

The Islamia University of Bahwalpur-Pakistan.

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