Skip to main content

Plant Pathology Introduction-Plant Care From Biotic & Abiotic

Plant Pathology Introduction-Plant Care From Biotic & Abiotic

PLANT PATHOLOGY INTRODUCTION:
Plant pathology is a science which covers all the aspects related to diseased plants such as causal organisms, their life cycle, diagnosis, physiology, population dynamics and management. Any abnormal functioning in the physiology of plants is designated as “disease”. This may be caused by biotic factors or “disorder” due to Abiotic factors. Generally these two words can be used alternatively. Biotic and Abiotic factors have been classified as follows:
BIOTIC

  • Prokaryotes
  • Fungi
  • Nematode
  • Virus Viriods Mycoplasma

ABIOTIC

  • Temperature Light
  • Nutritional deficiency
  • pH extreme
  • Mineral imbalance Wind
  • Pollutants
Plant Pathology Introduction-Plant Care From Biotic & Abiotic


DISEASE OR DISORDER:
All the biotic factors include microscopic and submicroscopic organisms. Followings are some terms related to study of microorganisms:
PARASITES (BIOTROPHS):
These are the microorganisms which take their nutrition from living organisms.
PATHOGENS:
These are the parasites which cause diseases in humans, animals and plants. Higher Plants
OBLIGATE PARASITES:
These are parasites which strictly live only on living organism for their nutrition and multiplication.
SAPROPHYTES:
These are microorganisms which get their nutrition from dead organic matter. These are also known as saprotrophs.
OBLIGATE SAPROPHYTES:
These are microorganisms which live only on dead organic matter to obtain their nutrition.
FACULTATIVE SAPROPHYTES:
The organisms which are originally parasites but can live saprophytically i.e. on dead organic matter in the absence of living host.
FACULTATIVE PARASITE:
The organisms which are originally saprophyte but can get nutrition from living host in the absence of dead organic matter.
SYMBIOSIS:
Mutual beneficial relation of host and parasite.
ANTAGONISM:
Counter action of two microorganisms against each other e.g. fungus Trichoderma harzianum is antagonist to many fungi. 
SYNERGISM:
It is the combined effect of two microorganisms on host plant.
PATHOGENESIS:
All the disease events in host plant starting from infection to development of symptoms.
INFESTATION:
It is just the presence of microorganism on the surface of the host.
INFECTION:
When pathogens get entry, start getting nutrition and disturb the normal
physiology of the plant.
SYMPTOM:
Physiological expression of the host as result of infection is called symptom.
Plant Pathology Introduction-Plant Care From Biotic & Abiotic

BRIEF DESCRIPTION OF PLANT PATHOGENS:
PATHOGEN
MORPHOLOGY
SIZE
SYMPTOMS
Fungi
Microscopic to Macroscopic, Branched, Filamentous, Somatic structure
Few micrometers to several meters in length. 0.5 μm-10 μm in diameters
Leaf spots, Gummosis, Powdery Mildew, Downy mildew, Rust, Smut, Blight, root rot of different crops.
Bacteria
Microscopic, Rod shaped (Bacillus), Spherical (Coccus), Comma shaped (Vibrio), Spiral (Spirillum)
0.6-3.5 μm in length 0.5-1.0 μm in diameter
Leaf spots, Blight, Canker, soft rot, Scab and Gall formation in vegetable and fruit crops.
Virus
Sub-microscopic, rod shaped, Spherical or polyhederal, flexous
480-2000 nm in length 10-13 nm wide Polyhedral viruses 17-60 nm in diameter
Yellowing, Mosaic, Mottling, Ring spot, Necrotic lesion, Curling, Rolling and Enation of leaves.
Dwarfing, Stunting of Plants
Nematode
Microscopic, eel shaped
300-1000 μm 4 mm in length 15-35 in width
Nutritional deficiency like symptoms, Gall Formation, Stunting, Rottening of roots, Bushy appearance of roots.

-------------------------------------------------------------------------------------------
Here You Can Find Plant Disease and Pathogens (Fungi, Bacteria, Molllicutes, Parasitic higher plants, Parasitic Green Algae, Nematodes, Protozoa, Viruses, Viroids,Prions) Diagnosis and Treatment, Control Against Plant Disease. Laboratory Experiments & Work. Plant Pathology Lab Manual. House Plant-Trees Care Guide, Snake Plant Care, Pothos Plant Care, Plant Care for dummies, Plant Care  Printable, Products, Plant Care Tips, Symbols, Tags, Database, Careers, Websites, Apps & Card.
Written By:
Dr.Qaiser Shakeel
Tahir Mahmood
Department of Plant Pathology-Agriculture
The Islamia University of Bahwalpur-Pakistan.

Comments

Popular posts from this blog

Preparation of Media For Culture-PDA, PDB & NA Recipes

PREPARATION OF MEDIA FOR CULTURE: Media play an important role not only for the nutrition and growth of the pathogen but also in identification through growth pattern on media. Fungi and Bacteria need synthetic media enriched with essential element for the growth. In general different types of media are prepared to culture fungi and bacteria but Potato dextrose agar (PDA) for fungi and Nutrient agar for bacteria are being used commonly.  RECIPES OF DIFFERENT MEDIA: POTATO DEXTROSE AGAR: Potato Starch 250  mL/15g Dextrose-15g Agar -Agar 15g Distilled water  750 mL/1000mL PROCEDURE TO PREPARE POTATO DEXTROSE AGAR MATERIAL: Petri dish, Flask, test tubes, Agar-agar, Dextrose, Potato starch, Water PROCEDURE: First of all take potatoes to extract potato starch. Peel the potatoes and cut into small pieces. Preferably 200 g of potato pieces are weighed, washed quickly in running water, placed in 1l liter of water and boiled for nearly

GEL Procedure Of Gel Electrophoresis For Separation DNA/RNA

GEL Procedure Of Gel Electrophoresis For Separation-DNA/RNA GEL ELECTROPHORESIS GEL Procedure Of Gel Electrophoresis For Separation-DNA/RNA Mix 1 g agarose in 99mL of TAE/TBE buffer and heat it in the oven. Add 10 uL of 10mg/mL ethidium bromide in 100 mL of hand cold agarose gel solution. Pour this mixture into gel caster/tray having comb and sealed from sides. Let it be solidified at room temperature for 30-60 minutes. Remove the comb seals from the gel caster and place it inside the gel tank containing TAE/TBE buffer. Put the DNA samples/PCR product (mixed in 6x loading dye) inside each well and note it in the lab. Notebook. Attach the cathode (towards wells) end of gel and anode (at the bottom) of the power supply to the gel tank. Switch on the power supply giving 80-120 volts and run it for sufficient time allowing the samples to travel in more than half of the gel length. Take the gel out of the gel tank and observe it in a gel doc system/UV transillumin

Total Plant RNA Isolation-Trizol Reagent Method

Total Plant RNA Isolation Using Trizol Reagent 1. Crush 100 mg of tissues in 1mL of triazole reagent by pestle mortar or micro pestle 1.5 or 2.0 mL RNase free Eppendorf tubes. *Incubate for 2-3 minutes at room temperature. 2. Add 200 uL chloroform and mix by gently inverting the tube. *Incubate for 2-3 minutes at room temperature. 3. Centrifuge at 12000xg at 4oC for 15 minutes. 4. Transfer the supernatant into fresh 1.5 mL RNA’s free tube. 5. Add 500 μL isopropanol mix gently by inverting the tube. *Incubate for 10 minutes at room temperature. 6. Centrifuge at 12000xg at 4oC for 10 minutes. *Discard the supernatant and keep the pallet in the tube. 7. Add 1mL of ice-chilled 75% ethanol to wash the pallet. 8. Centrifuge at 7500xg at 4oC for 5 minutes. *Discard the supernatant and keep the pallet in the tube. *Keep lid opened tube at 4oC for 15 min to air dry the pallet. 9. Add 50-100 uL RNA’s free water in pallet and mix by gentle pipetting. 10. Incubate for 10-15 m