Skip to main content

Generalized Life & Disease Cycle Of Plant Pathogenic Nematode-Plant Care



Generalized Life & Disease Cycle Of Plant Pathogenic Nematode-Plant Care
GENERALIZED LIFE CYCLE OF PLANT PARASITIC NEMATODES 

The nematodes are invertebrate roundworms that inhabit marine fresh water and terrestrial environments. The majority of nematodes are microscopic, averaging less than a millimeter in length, but some of the animal parasites are quite large and readily visible to the naked eye. The animal and plant parasites are of direct importance in agriculture, environment, human health; however, most nematodes in the environment are not parasites. Nematodes that feed on other organisms are important participants in the cycling of minerals and nutrients in the ecosystem that is fundamental to other biological activity. Some of these nematodes may have major roles in decomposition, including biodegradation of toxic compounds. Insect-parasitic nematodes can be of importance in regulating insect populations, and are being used in the biological control of insect pests e.g. Steinernema spp.
Generalized Life & Disease Cycle Of Plant Pathogenic Nematode-Plant Care

 Disease Cycle:
The J1 stage resides entirely inside the translucent egg case, where it molts into a J2 nematode. The motile J2 stage is the only stage that can initiate infections. J2s attack growing root tips and enter roots intercellularly. Nematode secretions cause dramatic physiological changes in the parasitized cells, transforming them into giant- cells. If the nematode dies, so will the giant-cells upon which it feeds. J2s do not possess reproductive organs. Root-knot nematodes undergo four juvenile stages, each progressing through a "molting" process similar to that of insects. As a result of this process, juvenile root-knot nematodes have little resemblance to adult males and females. In the J4 stage, the progression from juvenile to globose adult females or to vermiform adult males becomes clearly visible. They emerge as adults from the J4 cuticle. 
Generalized Life & Disease Cycle Of Plant Pathogenic Nematode-Plant Care
------------------------------------------------------------------------------------------
Here You Can Find Plant Disease and Pathogens (Fungi, Bacteria, Molllicutes, Parasitic higher plants, Parasitic Green Algae, Nematodes, Protozoa, Viruses, Viroids,Prions) Diagnosis and Treatment, Control Against Plant Disease. Laboratory Experiments & Work. Plant Pathology Lab Manual. House Plant-Trees Care Guide, Snake Plant Care, Pothos Plant Care, Plant Care for dummies, Plant Care  Printable, Products, Plant Care Tips, Symbols, Tags, Database, Careers, Websites, Apps & Card.
Written By:
Dr.Qaiser Shakeel
Tahir Mahmood
Department of Plant Pathology-Agriculture

The Islamia University of Bahwalpur-Pakistan.
Labels:

Comments

Post a Comment

Popular posts from this blog

Preparation of Media For Culture-PDA, PDB & NA Recipes

PREPARATION OF MEDIA FOR CULTURE: Media play an important role not only for the nutrition and growth of the pathogen but also in identification through growth pattern on media. Fungi and Bacteria need synthetic media enriched with essential element for the growth. In general different types of media are prepared to culture fungi and bacteria but Potato dextrose agar (PDA) for fungi and Nutrient agar for bacteria are being used commonly.  RECIPES OF DIFFERENT MEDIA: POTATO DEXTROSE AGAR: Potato Starch 250  mL/15g Dextrose-15g Agar -Agar 15g Distilled water  750 mL/1000mL PROCEDURE TO PREPARE POTATO DEXTROSE AGAR MATERIAL: Petri dish, Flask, test tubes, Agar-agar, Dextrose, Potato starch, Water PROCEDURE: First of all take potatoes to extract potato starch. Peel the potatoes and cut into small pieces. Preferably 200 g of potato pieces are weighed, washed quickly in running water, placed in 1l liter of water and boiled for nearly
Post a Comment
Read more

GEL Procedure Of Gel Electrophoresis For Separation DNA/RNA

GEL Procedure Of Gel Electrophoresis For Separation-DNA/RNA GEL ELECTROPHORESIS GEL Procedure Of Gel Electrophoresis For Separation-DNA/RNA Mix 1 g agarose in 99mL of TAE/TBE buffer and heat it in the oven. Add 10 uL of 10mg/mL ethidium bromide in 100 mL of hand cold agarose gel solution. Pour this mixture into gel caster/tray having comb and sealed from sides. Let it be solidified at room temperature for 30-60 minutes. Remove the comb seals from the gel caster and place it inside the gel tank containing TAE/TBE buffer. Put the DNA samples/PCR product (mixed in 6x loading dye) inside each well and note it in the lab. Notebook. Attach the cathode (towards wells) end of gel and anode (at the bottom) of the power supply to the gel tank. Switch on the power supply giving 80-120 volts and run it for sufficient time allowing the samples to travel in more than half of the gel length. Take the gel out of the gel tank and observe it in a gel doc system/UV transillumin
Post a Comment
Read more

Total Plant RNA Isolation-Trizol Reagent Method

Total Plant RNA Isolation Using Trizol Reagent 1. Crush 100 mg of tissues in 1mL of triazole reagent by pestle mortar or micro pestle 1.5 or 2.0 mL RNase free Eppendorf tubes. *Incubate for 2-3 minutes at room temperature. 2. Add 200 uL chloroform and mix by gently inverting the tube. *Incubate for 2-3 minutes at room temperature. 3. Centrifuge at 12000xg at 4oC for 15 minutes. 4. Transfer the supernatant into fresh 1.5 mL RNA’s free tube. 5. Add 500 μL isopropanol mix gently by inverting the tube. *Incubate for 10 minutes at room temperature. 6. Centrifuge at 12000xg at 4oC for 10 minutes. *Discard the supernatant and keep the pallet in the tube. 7. Add 1mL of ice-chilled 75% ethanol to wash the pallet. 8. Centrifuge at 7500xg at 4oC for 5 minutes. *Discard the supernatant and keep the pallet in the tube. *Keep lid opened tube at 4oC for 15 min to air dry the pallet. 9. Add 50-100 uL RNA’s free water in pallet and mix by gentle pipetting. 10. Incubate for 10-15 m
Post a Comment
Read more