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Equipments / Miscellaneous Lab Items Frequently Used In Plant Pathology Laboratory-Plant Care

EQUIPMENTS / MISCELLANEOUS LAB ITEMS FREQUENTLY USED IN PLANT PATHOLOGY LABORATORY

 EQUIPMENTS / MISCELLANEOUS LAB ITEMS FREQUENTLY USED IN PLANT PATHOLOGY LABORATORY
SR. No.
EQUIPMENTS/LAB ITEM
PURPOSE
1
Spirit lamp or gas burner
For sterilization of inoculation needles, forceps, scalpel, etc.
2
Autoclave
For sterilization of heat stable liquids — common culture media, dry bulk materials, heat resistant instruments, glassware.
3
Koch’s steam
Used for sterilization of media sterilizer or constituents which are damaged by exposure to temperature above 100°C, namely sugar, gelatin, milk.
4
Oven
For sterilization of glassware and metal instruments resistant to high temperature.
5
Gas sterilants
For sterilizing heat sensitive equipments and substrates
6
Liquid filters
For sterilization of heat sensitive solutions
7
Air filters
For sterilization of air for cultures and transfer chambers
8
Liquid disinfectants
Use on working surfaces, instruments, tools, etc
9
Laminar Flow Cabinet
It’s a hood to avoid contamination
10
Centrifuge Machine
Used to run a solution at high revolution to settle the small particles in solution
11
Hot Plate
Used to warm the liquids and media
12
Liquid Shaker
Used to culture the microbes in liquid media
13
Digital Balance
Used to weigh the small quantities of materials
14
Incubator
Used to optimize the conditions for the growth of cultures
15
Refrigerators
Used to store the specimens and materials at low temperatures
EQUIPMENTS / MISCELLANEOUS LAB ITEMS FREQUENTLY USED IN PLANT PATHOLOGY LABORATORY
EQUIPMENTS / MISCELLANEOUS LAB ITEMS FREQUENTLY USED IN PLANT PATHOLOGY LABORATORY

DISINFECTION AND STERILIZATION
The basic knowledge of disinfection and sterilization is important for biosafety in the laboratory to handle the contaminated materials.
EQUIPMENTS / MISCELLANEOUS LAB ITEMS FREQUENTLY USED IN PLANT PATHOLOGY LABORATORY

DEFINITIONS: The following are the commonly used terms for disinfection and sterilization.
ANTIMICROBIAL: An agent that kills microorganisms or suppresses their growth and multiplication.
ANTISEPTIC: A substance that inhibits the growth and development of microorganisms without necessarily killing them. Antiseptics are usually applied to body surfaces. 
BIOCIDE: A general term for any agent that kills organisms.
CHEMICAL GERMICIDE: A chemical or a mixture of chemicals used to kill microorganisms.
DECONTAMINATION: Any process for removing and/or killing microorganisms. The same term is also used for removing or neutralizing hazardous chemicals and radioactive materials. 
DISINFECTANT: A chemical or mixture of chemicals used to kill microorganisms, but not necessarily spores. Disinfectants are usually applied to inanimate surfaces or objects. 
DISINFECTION: A physical or chemical means of killing microorganisms, but not necessarily spores.
MICROBICIDE: A chemical or mixture of chemicals that kills microorganisms. The term is often used in place of “biocide”, “chemical germicide” or “antimicrobial”.
SPOROCIDE: A chemical or mixture of chemicals used to kill microorganisms and spores.
STERILIZATION: A process that kills and/or removes all classes of microorganisms and spores.
CHEMICAL DISINFECTION
The following chemicals or germicides are used to disinfect the materials.

  1. Chlorine (Sodium hypochlorite) Chloramines
  2. Chlorine dioxide (ClO2) Formaldehyde
  3. Alcohols
  4. Iodine and Iodophors Hydrogen peroxide Peracid
HEAT DISINFECTION AND STERILIZATION:
EQUIPMENTS / MISCELLANEOUS LAB ITEMS FREQUENTLY USED IN PLANT PATHOLOGY LABORATORY
Saturated steam under pressure (autoclaving) is the most effective way of sterilizing laboratory materials.
The following cycle will surely sterilize the correctly loaded materials.
  1. 3 minutes holding time at 134oC
  2. 10 minutes holding time at 126oC
  3. 15 minutes holding time at 121oC
  4. 25 minutes holding time at 115oC 
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Here You Can Find Plant Disease and Pathogens (Fungi, Bacteria, Molllicutes, Parasitic higher plants, Parasitic Green Algae, Nematodes, Protozoa, Viruses, Viroids,Prions) Diagnosis and Treatment, Control Against Plant Disease. Laboratory Experiments & Work. Plant Pathology Lab Manual. House Plant-Trees Care Guide, Snake Plant Care, Pothos Plant Care, Plant Care for dummies, Plant Care  Printable, Products, Plant Care Tips, Symbols, Tags, Database, Careers, Websites, Apps & Card.
Written By:
Dr.Qaiser Shakeel
Tahir Mahmood
Department of Plant Pathology-Agriculture
The Islamia University of Bahwalpur-Pakistan.
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